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rabbit anti-adrb2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit anti-adrb2
    Rabbit Anti Adrb2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-adrb2/adrb2+taqman+probe/us11857600-741-18-21
    Average 90 stars, based on 1 article reviews
    rabbit anti-adrb2 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: To perform PLA, PDCs were fixed with 4% paraformaldehyde (PFA) and were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PA5-33333), Rabbit anti-CHRM1 (1:200, Ls bio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2(1:200, Thermoscientific, PA5-33333), Rabbit anti-CHRM1(1:200, Ls bio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PA5-33333) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santa Cruz, Sc-53534), rabbit anti-ADRB2 (1:200, Thermo Scientific, PA5-33333), rabbit anti-CHRM1 (1:200, Lsbio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PAS-33333) at 37° C. for 1 h in a humidifying chamber.

    Incubation:

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: To perform PLA, PDCs were fixed with 4% paraformaldehyde (PFA) and were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PA5-33333), Rabbit anti-CHRM1 (1:200, Ls bio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2(1:200, Thermoscientific, PA5-33333), Rabbit anti-CHRM1(1:200, Ls bio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PA5-33333) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santa Cruz, Sc-53534), rabbit anti-ADRB2 (1:200, Thermo Scientific, PA5-33333), rabbit anti-CHRM1 (1:200, Lsbio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PAS-33333) at 37° C. for 1 h in a humidifying chamber.

    Proximity Ligation Assay:

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: To perform PLA, PDCs were fixed with 4% paraformaldehyde (PFA) and were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PA5-33333), Rabbit anti-CHRM1 (1:200, Ls bio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2(1:200, Thermoscientific, PA5-33333), Rabbit anti-CHRM1(1:200, Ls bio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PA5-33333) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santa Cruz, Sc-53534), rabbit anti-ADRB2 (1:200, Thermo Scientific, PA5-33333), rabbit anti-CHRM1 (1:200, Lsbio, Ls-C313301) at 37° C. for 1 h in a humidifying chamber.

    Article Title: GPCR heteromer inhibitors and uses thereof
    Article Snippet: Slides were blocked with blocking solution provided by Duolink and incubated with mouse anti-CXCR4 (1:200, Santacruz, Sc-53534), Rabbit anti-ADRB2 (1:200, Thermoscientific, PAS-33333) at 37° C. for 1 h in a humidifying chamber.



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    Glioblastomas alter their adrenoceptor expression levels depending on their stage of differentiation. (A). Number of RNA sequence reads representing the expression of mRNAs encoding adrenoceptors in GSCs and DGCs of MGG4, MGG6, and MGG8. ADRA1D , ADRA2C , ADRB1 , and <t>ADRB2</t> , which are well expressed and particularly variable, are highlighted with orange lines. (B). Graph showing the relative expression levels of ADRA1D , ADRA2C , ADRB1 , and ADRB2 in GSCs and DGCs of MGG4, MGG6, and MGG8 as a ratio of DGCs to GSCs. The vertical axis is shown in logarithm; of the four genes, those representing a common pattern of variation in the three cell lines are highlighted with green lines. (C). Western blotting results evaluating protein expression of ADRA1D, ADRB1, and ADRB2 in GSCs and DGCs of MGG4, MGG8, MGG18, and MGG23. (D). Immunostaining images showing changes in ADRA1D expression in GSCs and DGCs of MGG8. (E). Immunostaining image showing ADRA1D expression in tumor tissue of orthotopic xenografts composed of 8GSC-RFP and 8DGC-GFP. ADRA1D, which is upregulated in the differentiated state, is positive in some RFP-positive cells (formerly GSC cells) and negative in some GFP-positive cells (formerly DGC cells), indicating that, like undifferentiated and differentiated markers, the expression pattern of ADRA1D is also heterogeneous. Staining for ADRA1D in normal mouse brain tissue is shown as a control.
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    Glioblastomas alter their adrenoceptor expression levels depending on their stage of differentiation. (A). Number of RNA sequence reads representing the expression of mRNAs encoding adrenoceptors in GSCs and DGCs of MGG4, MGG6, and MGG8. ADRA1D , ADRA2C , ADRB1 , and <t>ADRB2</t> , which are well expressed and particularly variable, are highlighted with orange lines. (B). Graph showing the relative expression levels of ADRA1D , ADRA2C , ADRB1 , and ADRB2 in GSCs and DGCs of MGG4, MGG6, and MGG8 as a ratio of DGCs to GSCs. The vertical axis is shown in logarithm; of the four genes, those representing a common pattern of variation in the three cell lines are highlighted with green lines. (C). Western blotting results evaluating protein expression of ADRA1D, ADRB1, and ADRB2 in GSCs and DGCs of MGG4, MGG8, MGG18, and MGG23. (D). Immunostaining images showing changes in ADRA1D expression in GSCs and DGCs of MGG8. (E). Immunostaining image showing ADRA1D expression in tumor tissue of orthotopic xenografts composed of 8GSC-RFP and 8DGC-GFP. ADRA1D, which is upregulated in the differentiated state, is positive in some RFP-positive cells (formerly GSC cells) and negative in some GFP-positive cells (formerly DGC cells), indicating that, like undifferentiated and differentiated markers, the expression pattern of ADRA1D is also heterogeneous. Staining for ADRA1D in normal mouse brain tissue is shown as a control.
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    a Heatmaps of RNA-sequencing results. R and W represent the 2D ES and non-ES groups, respectively. <t>Adrb2</t> was significantly enriched compared with the other adrenergic receptor genes. b Volcano plots of candidate DEGs in the microarray datasets based on the screening criteria. Adrb2 showed no change between the non-ES and 2D ES groups. c Schematic of the intersectional genetic strategy used to generate Lyz2-Cre::Adrb2(f/f) mice. The statistical test was Wald test. d The percentage of ADRB2 + CD68 + in the CD68 + cells. These results verified the ablation of ADRB2 on monocytes/macrophages. n = 6 mice; two-sided Student’s unpaired t test; **** p < 0.0001. e ELISA for IL-6 in the non-ES and 2D ES groups of Lyz2-Cre::Adrb2(f/f) and Adrb2(f/f) mice. The decrease of IL-6 was prevented after 2D ES in Lyz2-Cre::Adrb2(f/f) mice. n = 6 mice; two-way ANOVA; F 1,20 = 63.53, p < 0.0001; p ost hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.2134. f Immunostaining of DAPI, IL-6 and CD68. Scale bar: 100 μm. g The percentage of IL-6 + CD68 + cells among CD68 + cells. The results were in accordance with the ELISA data. n = 6 mice; two-way ANOVA; F 1,20 = 58.85, p < 0.0001; post hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.9986. ES: electro-stimulation, non-ES: tendon surgery and flexible electrode implantation without ES group, 2D ES: tendon surgery, flexible electrode implantation and neuromorphic ES based on 2D FGM IDC group, ns: no significance. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Source data are provided as a file.
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    Image Search Results


    (A) ADRB2 expression in TCGA cancer tissues compared with corresponding TCGA and GTEx normal tissues. (B) ADRB2 expression in 7 types of cancer based on TCGA cancer and normal sample data. (C) Association between overall survival and ADRB2 expression in LUAD. (D) Immunoblot image of ADRB2 in LUAD and LUSC cell lines. ADRB2, adrenoceptor β2; GTEx, Genotype-Tissue Expression; TCGA, The Cancer Genome Atlas. * represents P < 0.05, ** represents P < 0.01, and *** represents P < 0.001.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: (A) ADRB2 expression in TCGA cancer tissues compared with corresponding TCGA and GTEx normal tissues. (B) ADRB2 expression in 7 types of cancer based on TCGA cancer and normal sample data. (C) Association between overall survival and ADRB2 expression in LUAD. (D) Immunoblot image of ADRB2 in LUAD and LUSC cell lines. ADRB2, adrenoceptor β2; GTEx, Genotype-Tissue Expression; TCGA, The Cancer Genome Atlas. * represents P < 0.05, ** represents P < 0.01, and *** represents P < 0.001.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques: Expressing, Western Blot

    (A) Percentages of wt, del and amp in LUAD. (B) Correlation between copy number variations and ADRB2 mRNA expression in LUAD samples. (C) Correlation between amp and ADRB2 mRNA expression in LUAD samples. (D) Correlation between dels and ADRB2 mRNA expression in LUAD samples. (E) Correlation between DNA methylation and ADRB2 mRNA in LUAD samples. (F) miRNA-ADRB2 regulatory network generated using Cytoscape software. (G) Examination of miR-424-5p expression in LUAD and normal samples conducted using the StarBase database. (H) Result of dual luciferase reporter gene experiment. ADRB2, adrenoceptor β2; amp, amplification; del, deletion; LUAD, lung adenocarcinoma; miR/miRNA, microRNA; wt, wild-type.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: (A) Percentages of wt, del and amp in LUAD. (B) Correlation between copy number variations and ADRB2 mRNA expression in LUAD samples. (C) Correlation between amp and ADRB2 mRNA expression in LUAD samples. (D) Correlation between dels and ADRB2 mRNA expression in LUAD samples. (E) Correlation between DNA methylation and ADRB2 mRNA in LUAD samples. (F) miRNA-ADRB2 regulatory network generated using Cytoscape software. (G) Examination of miR-424-5p expression in LUAD and normal samples conducted using the StarBase database. (H) Result of dual luciferase reporter gene experiment. ADRB2, adrenoceptor β2; amp, amplification; del, deletion; LUAD, lung adenocarcinoma; miR/miRNA, microRNA; wt, wild-type.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques: Expressing, DNA Methylation Assay, Generated, Software, Luciferase, Amplification

    (A) Various immune cells infiltrate LUAD levels in case of different copy numbers of ADRB2. (B) Correlation of ADRB2 expression with infiltration levels of immune cells in LUAD. (C) and (D) ADRB2 expression specifically within these immune cell types at the single-cell level. (E) Chemokine receptor genes. (F) Chemokine genes. (G) Immune activation genes. (H) Immunosuppressive genes. ADRB2, adrenoceptor β2; LUAD, lung adenocarcinoma. Correlation between adrenoceptor β2 and tumor immunity-related genes.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: (A) Various immune cells infiltrate LUAD levels in case of different copy numbers of ADRB2. (B) Correlation of ADRB2 expression with infiltration levels of immune cells in LUAD. (C) and (D) ADRB2 expression specifically within these immune cell types at the single-cell level. (E) Chemokine receptor genes. (F) Chemokine genes. (G) Immune activation genes. (H) Immunosuppressive genes. ADRB2, adrenoceptor β2; LUAD, lung adenocarcinoma. Correlation between adrenoceptor β2 and tumor immunity-related genes.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques: Expressing, Single Cell, Activation Assay

    (A) Validation of stable ADRB2 gene overexpression in LUAD Cell lines. (B) Comparison of tumor volume between control and ADRB2-overexpressing groups. (C) Visualization of tumorigenesis via small animal live imaging. (D) Comparison of tumor size between the control and ADRB2-overexpressing groups in C57BL/6 mice. Tumors in mice with ADRB2-overexpressing LUAD cells were significantly smaller, indicating the suppressive effect of ADRB2 on tumor growth. (E) Flow cytometry analysis of CD4 + T cell infiltration in tumor tissues from C57BL/6 mice (CD45 + CD11b - CD45R - CD3 + CD4 + ). (F) Flow cytometry analysis of dendritic cell infiltration in tumor tissues from C57BL/6 mice (CD45 + CD11c + MHC II + ). ** P < 0.01, *** P < 0.01. ADRB2, adrenoceptor β2.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: (A) Validation of stable ADRB2 gene overexpression in LUAD Cell lines. (B) Comparison of tumor volume between control and ADRB2-overexpressing groups. (C) Visualization of tumorigenesis via small animal live imaging. (D) Comparison of tumor size between the control and ADRB2-overexpressing groups in C57BL/6 mice. Tumors in mice with ADRB2-overexpressing LUAD cells were significantly smaller, indicating the suppressive effect of ADRB2 on tumor growth. (E) Flow cytometry analysis of CD4 + T cell infiltration in tumor tissues from C57BL/6 mice (CD45 + CD11b - CD45R - CD3 + CD4 + ). (F) Flow cytometry analysis of dendritic cell infiltration in tumor tissues from C57BL/6 mice (CD45 + CD11c + MHC II + ). ** P < 0.01, *** P < 0.01. ADRB2, adrenoceptor β2.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques: Biomarker Discovery, Over Expression, Comparison, Control, Imaging, Flow Cytometry

    (A) Correlation of ADRB2 and PD-1 expression in LUAD. (B) Correlation of ADRB2 and PD-L1 expression in LUAD. (C) Correlation of ADRB2 and CTLA-4 expression in LUAD. (D) The correlation of ADRB2 and PD-1 expression in LUAD was verified using the GEPIA database. (E) The correlation of ADRB2 and PD-L1 expression in LUAD was verified using the GEPIA database. (F) The correlation of ADRB2 and CTLA-4 expression in LUAD was verified using the GEPIA database. ADRB2, adrenoceptor β2; CTLA-4, cytotoxic T-lymphocyte associated protein 4; GEPIA, Gene Expression Profiling Interactive Analysis; LUAD, lung adenocarcinoma; PD-1, programmed cell death protein 1; PD-L1, programmed death-ligand 1.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: (A) Correlation of ADRB2 and PD-1 expression in LUAD. (B) Correlation of ADRB2 and PD-L1 expression in LUAD. (C) Correlation of ADRB2 and CTLA-4 expression in LUAD. (D) The correlation of ADRB2 and PD-1 expression in LUAD was verified using the GEPIA database. (E) The correlation of ADRB2 and PD-L1 expression in LUAD was verified using the GEPIA database. (F) The correlation of ADRB2 and CTLA-4 expression in LUAD was verified using the GEPIA database. ADRB2, adrenoceptor β2; CTLA-4, cytotoxic T-lymphocyte associated protein 4; GEPIA, Gene Expression Profiling Interactive Analysis; LUAD, lung adenocarcinoma; PD-1, programmed cell death protein 1; PD-L1, programmed death-ligand 1.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques: Expressing, Gene Expression

    Data from the GDSC shows the association between ADRB2 expression and drug sensitivity.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: Data from the GDSC shows the association between ADRB2 expression and drug sensitivity.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques: Expressing

    ADRB2, adrenoceptor β2; miR, microRNA.

    Journal: PLOS One

    Article Title: C1RL-AS1/microRNA-424-5p/adrenoceptor β2 axis: A novel regulatory mechanism in lung adenocarcinoma associated with immune infiltration and prognosis

    doi: 10.1371/journal.pone.0343805

    Figure Lengend Snippet: ADRB2, adrenoceptor β2; miR, microRNA.

    Article Snippet: ADRB2 was determined with the primary anti-ADRB2 antibody (29864–1-AP, Wuhan Proteintech Group, Inc., Ltd) and secondary antibody (SA00001–2, Wuhan Proteintech Group, Inc., Ltd).

    Techniques:

    Glioblastomas alter their adrenoceptor expression levels depending on their stage of differentiation. (A). Number of RNA sequence reads representing the expression of mRNAs encoding adrenoceptors in GSCs and DGCs of MGG4, MGG6, and MGG8. ADRA1D , ADRA2C , ADRB1 , and ADRB2 , which are well expressed and particularly variable, are highlighted with orange lines. (B). Graph showing the relative expression levels of ADRA1D , ADRA2C , ADRB1 , and ADRB2 in GSCs and DGCs of MGG4, MGG6, and MGG8 as a ratio of DGCs to GSCs. The vertical axis is shown in logarithm; of the four genes, those representing a common pattern of variation in the three cell lines are highlighted with green lines. (C). Western blotting results evaluating protein expression of ADRA1D, ADRB1, and ADRB2 in GSCs and DGCs of MGG4, MGG8, MGG18, and MGG23. (D). Immunostaining images showing changes in ADRA1D expression in GSCs and DGCs of MGG8. (E). Immunostaining image showing ADRA1D expression in tumor tissue of orthotopic xenografts composed of 8GSC-RFP and 8DGC-GFP. ADRA1D, which is upregulated in the differentiated state, is positive in some RFP-positive cells (formerly GSC cells) and negative in some GFP-positive cells (formerly DGC cells), indicating that, like undifferentiated and differentiated markers, the expression pattern of ADRA1D is also heterogeneous. Staining for ADRA1D in normal mouse brain tissue is shown as a control.

    Journal: The Journal of Physiological Sciences : JPS

    Article Title: Changes in adrenoceptor expression level contribute to the cellular plasticity of glioblastoma cells

    doi: 10.1016/j.jphyss.2025.100016

    Figure Lengend Snippet: Glioblastomas alter their adrenoceptor expression levels depending on their stage of differentiation. (A). Number of RNA sequence reads representing the expression of mRNAs encoding adrenoceptors in GSCs and DGCs of MGG4, MGG6, and MGG8. ADRA1D , ADRA2C , ADRB1 , and ADRB2 , which are well expressed and particularly variable, are highlighted with orange lines. (B). Graph showing the relative expression levels of ADRA1D , ADRA2C , ADRB1 , and ADRB2 in GSCs and DGCs of MGG4, MGG6, and MGG8 as a ratio of DGCs to GSCs. The vertical axis is shown in logarithm; of the four genes, those representing a common pattern of variation in the three cell lines are highlighted with green lines. (C). Western blotting results evaluating protein expression of ADRA1D, ADRB1, and ADRB2 in GSCs and DGCs of MGG4, MGG8, MGG18, and MGG23. (D). Immunostaining images showing changes in ADRA1D expression in GSCs and DGCs of MGG8. (E). Immunostaining image showing ADRA1D expression in tumor tissue of orthotopic xenografts composed of 8GSC-RFP and 8DGC-GFP. ADRA1D, which is upregulated in the differentiated state, is positive in some RFP-positive cells (formerly GSC cells) and negative in some GFP-positive cells (formerly DGC cells), indicating that, like undifferentiated and differentiated markers, the expression pattern of ADRA1D is also heterogeneous. Staining for ADRA1D in normal mouse brain tissue is shown as a control.

    Article Snippet: ADRB2 Rabbit Poly Ab , 1:2000 , Proteintech , 13096 −1-AP.

    Techniques: Expressing, Sequencing, Western Blot, Immunostaining, Staining, Control

    The antibodies used for Western blotting.

    Journal: The Journal of Physiological Sciences : JPS

    Article Title: Changes in adrenoceptor expression level contribute to the cellular plasticity of glioblastoma cells

    doi: 10.1016/j.jphyss.2025.100016

    Figure Lengend Snippet: The antibodies used for Western blotting.

    Article Snippet: ADRB2 Rabbit Poly Ab , 1:2000 , Proteintech , 13096 −1-AP.

    Techniques: Western Blot, Produced

    a Heatmaps of RNA-sequencing results. R and W represent the 2D ES and non-ES groups, respectively. Adrb2 was significantly enriched compared with the other adrenergic receptor genes. b Volcano plots of candidate DEGs in the microarray datasets based on the screening criteria. Adrb2 showed no change between the non-ES and 2D ES groups. c Schematic of the intersectional genetic strategy used to generate Lyz2-Cre::Adrb2(f/f) mice. The statistical test was Wald test. d The percentage of ADRB2 + CD68 + in the CD68 + cells. These results verified the ablation of ADRB2 on monocytes/macrophages. n = 6 mice; two-sided Student’s unpaired t test; **** p < 0.0001. e ELISA for IL-6 in the non-ES and 2D ES groups of Lyz2-Cre::Adrb2(f/f) and Adrb2(f/f) mice. The decrease of IL-6 was prevented after 2D ES in Lyz2-Cre::Adrb2(f/f) mice. n = 6 mice; two-way ANOVA; F 1,20 = 63.53, p < 0.0001; p ost hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.2134. f Immunostaining of DAPI, IL-6 and CD68. Scale bar: 100 μm. g The percentage of IL-6 + CD68 + cells among CD68 + cells. The results were in accordance with the ELISA data. n = 6 mice; two-way ANOVA; F 1,20 = 58.85, p < 0.0001; post hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.9986. ES: electro-stimulation, non-ES: tendon surgery and flexible electrode implantation without ES group, 2D ES: tendon surgery, flexible electrode implantation and neuromorphic ES based on 2D FGM IDC group, ns: no significance. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Neuromorphic electro-stimulation based on atomically thin semiconductor for damage-free inflammation inhibition

    doi: 10.1038/s41467-024-45590-8

    Figure Lengend Snippet: a Heatmaps of RNA-sequencing results. R and W represent the 2D ES and non-ES groups, respectively. Adrb2 was significantly enriched compared with the other adrenergic receptor genes. b Volcano plots of candidate DEGs in the microarray datasets based on the screening criteria. Adrb2 showed no change between the non-ES and 2D ES groups. c Schematic of the intersectional genetic strategy used to generate Lyz2-Cre::Adrb2(f/f) mice. The statistical test was Wald test. d The percentage of ADRB2 + CD68 + in the CD68 + cells. These results verified the ablation of ADRB2 on monocytes/macrophages. n = 6 mice; two-sided Student’s unpaired t test; **** p < 0.0001. e ELISA for IL-6 in the non-ES and 2D ES groups of Lyz2-Cre::Adrb2(f/f) and Adrb2(f/f) mice. The decrease of IL-6 was prevented after 2D ES in Lyz2-Cre::Adrb2(f/f) mice. n = 6 mice; two-way ANOVA; F 1,20 = 63.53, p < 0.0001; p ost hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.2134. f Immunostaining of DAPI, IL-6 and CD68. Scale bar: 100 μm. g The percentage of IL-6 + CD68 + cells among CD68 + cells. The results were in accordance with the ELISA data. n = 6 mice; two-way ANOVA; F 1,20 = 58.85, p < 0.0001; post hoc Tukey test: *** p < 0.001; **** p < 0.0001; ns, p = 0.9986. ES: electro-stimulation, non-ES: tendon surgery and flexible electrode implantation without ES group, 2D ES: tendon surgery, flexible electrode implantation and neuromorphic ES based on 2D FGM IDC group, ns: no significance. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Source data are provided as a file.

    Article Snippet: The primary antibodies used in this study included the following: rat anti-CD68 (NBP2-33337, Novus Biologicals; 1:400), rabbit anti-IL-6 (ab290735, Abcam; 1:50), rabbit anti-ADRB2 (ab182136, Abcam; 1:100), rabbit anti-Cleaved Caspase-3 (Cat. # 9661 S, CST; 1:400).

    Techniques: RNA Sequencing Assay, Microarray, Enzyme-linked Immunosorbent Assay, Immunostaining